{rfName}
AF

Indexed in

License and use

Citations

7

Altmetrics

Analysis of institutional authors

Jorba IAuthorLuque TAuthorNavajas D.Author
Share
Publications
>
Article

AFM and microrheology in the zebrafish embryo yolk cell

Publicated to:Jove-Journal Of Visualized Experiments. (129): e56224- - 2017-11-29 (129), DOI: 10.3791/56224

Authors: Marsal, M; Jorba, I; Rebollo, E; Luque, T; Navajas, D; Martín-Blanco, E

Affiliations

Cellular and Respiratory Biomechanics. Institute for Bioengineering of Catalonia - Author
Centro de Investigacion Biomedica en Red de Enfermedades Respiratorias - Author
Ciber Enfermedades Resp, Barcelona, Spain - Author
CSIC - Instituto de Biologia Molecular de Barcelona (IBMB) - Author
CSIC, Inst Biol Mol Barcelona, Madrid, Spain - Author
Institute for Bioengineering of Catalonia - Author
Univ Barcelona, Inst Bioengn Catalonia, Barcelona, Spain - Author
See more

Abstract

© 2017 Journal of Visualized Experiments. Elucidating the factors that direct the spatio-temporal organization of evolving tissues is one of the primary purposes in the study of development. Various propositions claim to have been important contributions to the understanding of the mechanical properties of cells and tissues in their spatiotemporal organization in different developmental and morphogenetic processes. However, due to the lack of reliable and accessible tools to measure material properties and tensional parameters in vivo, validating these hypotheses has been difficult. Here we present methods employing atomic force microscopy (AFM) and particle tracking with the aim of quantifying the mechanical properties of the intact zebrafish embryo yolk cell during epiboly. Epiboly is an early conserved developmental process whose study is facilitated by the transparency of the embryo. These methods are simple to implement, reliable, and widely applicable since they overcome intrusive interventions that could affect tissue mechanics. A simple strategy was applied for the mounting of specimens, AFM recording, and nanoparticle injections and tracking. This approach makes these methods easily adaptable to other developmental times or organisms.

Keywords
Atomic force microscopyCortical tensionDevelopmental biologyIssue 129MicrorheologyNanoparticle trackingYolkZebrafish

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Jove-Journal Of Visualized Experiments due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2017, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Chemical Engineering (Miscellaneous).

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-04-25:

  • WoS: 1
  • Scopus: 6
  • OpenCitations: 1
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-04-25:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 20.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 20 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 0.25.

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.