Fibronectin/thermo-responsive polymer scaffold as a dynamic ex vivo niche for mesenchymal stem cells

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Publicated to:Journal Of Materials Science-Materials In Medicine. 31 (12): 129- - 2020-12-01 31(12), DOI: 10.1007/s10856-020-06461-y

Authors: Ramalho, L; Nedjari, S; Guarino, R; Awaja, F; Gugutkov, D; Altankov, G

Affiliations

Bulgarian Acad Sci, Inst Biophys & Biomed Engn, Sofia, Bulgaria - Author

Bulgarian Academy of Sciences - Author

Ecole Polytech Fed Lausanne, Swiss Plasma Ctr, CH-5232 Villigen, Switzerland - Author

Ecole Polytechnique Federale de Lausanne - Author

Engmat Ltd, Clybaun Rd, Galway, Ireland - Author

Engmat Ltd. - Author

ICREA, Barcelona, Spain - Author

Inst Bioengn Catalonia IBEC, Barcelona, Spain - Author

Institució Catalana de Recerca i Estudis Avançats - Author

Institute for Bioengineering of Catalonia - Author

Instituto de Bioingeniería de Cataluña - Author

Molecular Dynamics at Cell-Biomaterial Interface. Institute for Bioengineering of Catalonia - Author

National University of Ireland Galway - Author

Natl Univ Ireland, Ctr Res Med Devices CURAM, Galway, Ireland - Author

Natl Univ Ireland, Regenerat Med Inst REMEDI, Galway, Ireland - Author

Univ Lisbon, Inst Biophys & Biomed Engn, Fac Sci, Lisbon, Portugal - Author

UNIVERSIDADE DE LISBOA - Author

Abstract

© 2020, Springer Science+Business Media, LLC, part of Springer Nature. In this paper, we created a dynamic adhesive environment (DAE) for adipose tissue-derived mesenchymal stem cells (ADMSCs) cultured on smart thermo-responsive substrates, i.e., poly (N-isopropyl acrylamide) (PNIPAM), via introducing periodic changes in the culture temperature. We further explored the particular role of adsorbed fibronectin (FN), an important cell adhesive protein that was recently attributed to the recruitment of stem cells in the niche. The engineered FN/PNIPAM DAE system significantly increased the symmetric renewal of ADMSCs, particularly between passages 7 and 9 (p7–p9), before it dropped down to the level of the control (FN-coated TC polystyrene). This decline in the growth curve was consistent with the increased number of senescent cells, the augmented average cell size and the suppressed FN matrix secretion at late passages (p10–p12), all of them characteristic for stem cells ageing, which equivocally tended to slow down at our DAE system. FN supported also the osteogenic response of ADMSCs (apart from the previous observations with plain PNIPAM substrata) indicated by the significant increase of alkaline phosphatase (ALP) activity at days 7 and 14. The minimal changes in the Ca deposition, however, suggest a restricted effect of DAE on the early osteogenic response of ADMSCs only. Thus, the engineering of niche-like DAE involving FN uncovers a new tissue engineering strategy for gaining larger amounts of functionally active stem cells for clinical application. [Figure not available: see fulltext.]

Keywords

detachmentextracellular-matrixmuscleproliferation ratesenescencesizesurfacesvitronectinOsteogenic differentiation

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Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal Of Materials Science-Materials In Medicine due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2020, it was in position 33/89, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Engineering, Biomedical. Notably, the journal is positioned en el Cuartil Q2 para la agencia Scopus (SJR) en la categoría Biomedical Engineering.

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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Bulgaria; Portugal; Switzerland; United Kingdom.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (Altankov, George).

the author responsible for correspondence tasks has been Altankov, George.

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